Hydrodynamics-based liver transfection achieves gene silencing of CB1 using short hairpin RNA plasmid in cirrhotic rats
Díaz-Rivera, A., Meza-Ríos, A., Chagoya de Sánchez, V., Velasco-Loyden, G., García-Benavides, L., Jave-Suarez, L. F., ? Sandoval-Rodríguez, A. (2020). Hydrodynamics-based liver transfection achieves gene silencing of CB1 using short hairpin RNA plasmid in cirrhotic rats. PLOS ONE, 15(2), e0228729. doi:10.1371/journal.pone.0228729
ABSTRACT
There is a correlation between the endocannabinoid system and hepatic fibrosis based on the activation of CB1 and CB2 receptors; where CB1 has profibrogenic effects. Gene therapy with a plasmid carrying a shRNA for CB1 delivered by hydrodynamic injection has the advantage of hepatic tropism, avoiding possible undesirable effects of CB1 pharmacological inhibition.