The purpose of this study was to determine the effect of the implantation of Taenia solium metacestodes and the treatment with suppressive metacestode factor (F1) on the ability of spleen cells from Balb/c mice to produce cytokines. Cytokine production was estimated 12 days following the implantation or 4 days after the last dose of F1 (five doses) by RT-PCR and flow cytometry analyses. Spleen cells were obtained from metacestode-implanted, F1-treated and control mice. They were stimulated with concanavalin A ( ConA) ex vivo and used for RT-PCR studies and for CD25 expression and intracellular cytokine production estimations using specific monoclonal antibodies labeled with phycoerithrin or fluorescein. Results of the RTPCR showed that all cells expressed IFN-gamma, IL-2 and IL-4 mRNAs. IL-10 mRNA was not expressed in any case. Flow cytometry analyses showed that both spleen CD4(+) and CD8(+) cells from metacestode-implanted or treated-F1 mice expressed significantly diminished percentages of CD25 when compared with control cells ( P< 0.05). The estimation of intracellular cytokines showed that the production of IL-2 and IL-4 in CD8(+) cells, and of IFN-γ in CD4(+) cells from mice implanted with metacestodes was significantly impaired when compared with the values from control cells ( P< 0.05).
Última actualización: 22/03/2019