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Georgellis, D; Pena-Sandoval, GR (2010)

THE ARCB SENSOR KINASE OF ESCHERICHIA COLI AUTOPHOSPHORYLATES BY AN INTRAMOLECULAR REACTION

J BACTERIOL 192(6):1735-1739
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The Arc two-component system, comprising the ArcB sensor kinase and the ArcA response regulator, modulates the expression of numerous genes in response to the respiratory conditions of growth. ArcB is a tripartite histidine kinase whose activity is regulated by the oxidation of two cytosol-located redox-active cysteine residues that participate in intermolecular disulfide bond formation. Here we show that ArcB auto-phosphorylates through an intramolecular reaction which diverges from the usually envisaged intermolecular autophosphorylation of homodimeric histidine kinases.