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Rosas-Lemus, Monica; Uribe-Alvarez, Cristina; Chiquete-Felix, Natalia; Uribe-Carvajal, Salvador (2014)

IN SACCHAROMYCES CEREVISIAE FRUCTOSE-1,6-BISPHOSPHATE CONTRIBUTES TO THE CRABTREE EFFECT THROUGH CLOSURE OF THE MITOCHONDRIAL UNSPECIFIC CHANNEL

Arch. Biochem. Biophys. 555():66-70
full text

In Saccharomyces cerevisiae addition of glucose inhibits oxygen consumption, i.e. S. cerevisiae is Crabtree-positive. During active glycolysis hexoses-phosphate accumulate, and probably interact with mitochondria. In an effort to understand the mechanism underlying the Crabtree effect, the effect of two glycolysis-derived hexoses-phosphate was tested on the S. cerevisiae mitochondrial unspecific channel (ScMUC). Glucose-6-phosphate (G6P) promoted partial opening of ScMUC, which led to proton leakage and uncoupling which in turn resulted in, accelerated oxygen consumption. In contrast, fructose-1,6-bisphosphate (F1,6BP) closed ScMUC and thus inhibited the rate of oxygen consumption. When added together, F1,6BP reverted the mild G6P-induced effects. F1,6BP is proposed to be an important modulator of ScMUC, whose closure contributes to the "Crabtree effect".