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Aguilar-Roblero, R; Hernandez Munoz, R; Escobar, C; Mendoza, JY; Salazar-Juarez, A; Avila, J; Diaz-Munoz, M (2002)

RATS MADE CIRRHOTIC BY CHRONIC CCL4 TREATMENT STILL EXHIBIT ANTICIPATORY ACTIVITY TO A RESTRICTED FEEDING SCHEDULE

CHRONOBIOL INT 19(6):1073-1086
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Food entrainment of clock genes in the liver suggests that this organ may underlie a food entrained oscillator (FEO), which manifests under restricted feeding schedule (RFS). In order to test the importance of a functional liver for the expression of FEO, chronic CCl4-treated cirrhotic rats and oil-treated controls were entrained to RFS and drinking behavior was continuously monitored. After 20 d of free-running conditions, food access was restricted to 2 h, followed by a refeeding-fasting protocol to test persistence of anticipatory drinking. Present data show no differences between groups for the onset and intensity of anticipation during RFS. After RFS, however, cirrhotic rats exhibited a significantly longer free-running period and a delay and lower intensity of the persistence of activity under fasting conditions. Histology confirmed injury of the liver chronically treated with CCl4. Present data indicate that a dysfunctional liver due to chronic CCL4 does not present animals from exhibiting anticipatory activity but may promote metabolic derangement of the clock mechanisms of the suprachiasmatic nucleus and the FEO.