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Gomora, JC; Cribbs, LL; Daud, AN; Lee, JH; Perez-Reyes, E (2000)

MOLECULAR CLONING AND FUNCTIONAL EXPRESSION OF CA(V)3.1C, A T-TYPE CALCIUM CHANNEL FROM HUMAN BRAIN

FEBS LETT 466(1):54-58
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Low voltage-activated T-type calcium channels are encoded by a family of at least three genes, with additional diversity created by alternative splicing, This study describes the cloning of the human brain alpha 1G, which is a novel isoform, Ca(v)3.1c, Comparison of this sequence to genomic sequences deposited in the GenBank allowed us to identify the intron/exon boundaries of the human CACNA1G gene, A full-length cDNA mas constructed, then used to generate a stably-transfected mammalian cell line. The resulting currents were analyzed for their voltage- and time-dependent properties, These properties identify this gene as encoding a T-type Ca2+ channel. (C) 2000 Federation of European Biochemical Societies.