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Carmona-Aldana F; Nuñez-Martinez HN; Peralta-Alvarez CA; Tapia-Urzua G; Recillas-Targa F (2021)

GENERATION OF FUNCTIONAL GENETIC STUDY MODELS IN ZEBRAFISH USING CRISPR-CAS9

Methods in Molecular Biology 2174():255-262
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© 2021, Springer Science+Business Media, LLC, part of Springer Nature. CRISPR-Cas9 is a method for genome editing that can be used efficiently for in vivo applications; the basic implementation of this method is used to generate genome site-directed sequence eliminations. Here we describe a protocol for genome editing using CRISPR-Cas9 in zebrafish (Danio rerio) one-cell embryos.