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Perez Montfort, R; GomezPuyou A; Torres-Larios, A; Arreola, R; Vega-Miranda, A; Merino-Perez, E (2008)

EXPRESSION, PURIFICATION AND PRELIMINARY X-RAY DIFFRACTION STUDIES OF THE TRANSCRIPTIONAL FACTOR PYRR FROM BACILLUS HALODURANS

ACTA CRYSTALLOGR F 64():692-696
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The PyrR transcriptional regulator is widely distributed in bacteria. This RNA-binding protein is involved in the control of genes involved in pyrimidine biosynthesis, in which uridyl and guanyl nucleotides function as effectors. Here, the crystallization and preliminary X-ray diffraction analysis of two crystal forms of Bacillus halodurans PyrR are reported. One of the forms belongs to the monoclinic space group P2(1) with unit-cell parameters a = 59.7, b = 87.4, c = 72.1 angstrom, beta = 104.4 degrees, while the other form belongs to the orthorhombic space group P22(1)2(1) with unit-cell parameters a = 72.7, b = 95.9, c = 177.1 angstrom. Preliminary X-ray diffraction data analysis and molecular-replacement solution revealed the presence of four and six monomers per asymmetric unit; a crystallographic tetramer is formed in both forms.