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Garcia-Aguirre, Ian; Alamillo-Iniesta, Alma; Rodriguez-Perez, Ruth; Velez-Aguilera, Griselda; Amaro-Encarnacion, Elianeth; Jimenez-Gutierrez, Elizabeth; Vasquez-Limeta, Alejandra; Samuel Laredo-Cisneros, Marco; Morales-Lazaro, Sara L; Tiburcio-Felix, Reynaldo; Ortega, Arturo; Magana, Jonathan J; Winder, Steve J; Cisneros, Bulmaro (2019)

ENHANCED NUCLEAR PROTEIN EXPORT IN PREMATURE AGING AND RESCUE OF THE PROGERIA PHENOTYPE BY MODULATION OF CRM1 ACTIVITY

Aging Cell ():
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The study of Hutchinson-Gilford progeria syndrome (HGPS) has provided important clues to decipher mechanisms underlying aging. Progerin, a mutant lamin A, disrupts nuclear envelope structure/function, with further impairment of multiple processes that culminate in senescence. Here, we demonstrate that the nuclear protein export pathway is exacerbated in HGPS, due to progerin-driven overexpression of CRM1, thereby disturbing nucleocytoplasmic partitioning of CRM1-target proteins. Enhanced nuclear export is central in HGPS, since pharmacological inhibition of CRM1 alleviates all aging hallmarks analyzed, including senescent cellular morphology, lamin B1 downregulation, loss of heterochromatin, nuclear morphology defects, and expanded nucleoli. Exogenous overexpression of CRM1 on the other hand recapitulates the HGPS cellular phenotype in normal fibroblasts. CRM1 levels/activity increases with age in fibroblasts from healthy donors, indicating that altered nuclear export is a common hallmark of pathological and physiological aging. Collectively, our findings provide novel insights into HGPS pathophysiology, identifying CRM1 as potential therapeutic target in HGPS.