In order to determine whether follicle stimulating hormone (FSH) regulates P-450 aromatase (P-450 arom) in salmonid fish, we investigated the in vitro effects of FSH on estradiol (E 2 ) production and P-450 arom activity and expression in brown trout (Salmo trutta) vitellogenic ovarian follicles. Brown trout ovarian follicles were incubated in the presence of coho salmon FSH and the production of E 2 into the medium was measured by RIA, the activity of P-450 arom by the tritiated water release assay and the expression of P-450 arom by Northern blotting using a homologous cDNA probe obtained by RT-PCR. Results from this study indicate that the dose- and time-dependent stimulatory effects of FSH on E 2 production are dependent on new RNA and protein synthesis. The basal and FSH-stimulated E 2 production was completely blocked by fadrozole, a specific aromatase inhibitor. FSH was capable of stimulating P-450 arom activity but this stimulation was only detectable with short incubation times (30min) since longer incubation times with FSH resulted in the inhibition of P-450 arom activity. In addition, FSH increased the steady-state P-450 arom mRNA levels. In conclusion, our results indicate, for the first time in teleost fish, that FSH stimulates the expression of P-450 arom, as well as its activity, albeit after a short-term treatment with FSH, and that FSH plays a fundamental role in the regulation of the production of E 2 in the salmonid ovary. © 2004 Elsevier Inc. All rights reserved.
Última actualización: 23/07/2018