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Zarate-Romero, Andres; Stojanoff, Vivian; Patricia Rojas-Trejo, Sonia; Hansberg, Wilhelm; Rudino-Pinera, Enrique (2013)

CONFORMATIONAL STABILITY AND CRYSTAL PACKING: POLYMORPHISM IN NEUROSPORA CRASSA CAT-3

Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 69():753-758
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Polymorphism is frequently observed from different crystallization conditions. In proteins, the effect on conformational variability is poorly documented, with only a few reported examples. Here, three polymorphic crystal structures determined for a large-subunit catalase, CAT-3 from Neurospora crassa, are reported. Two of them belonged to new space groups, P1 and P43212, and a third structure belonged to the same space group, P212121, as the previously deposited 2.3?Å resolution structure (PDB entry 3ej6), but had a higher resolution (1.95?Å). Comparisons between these polymorphic structures highlight the conformational stability of tetrameric CAT-3 and reveal a distortion in the tetrameric structure that has not previously been described.