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Luis Velazquez-Velazquez, Jorge; Santos-Flores, Andres; Araujo-Melendez, Javier; Sanchez-Sanchez, Roberto; Velasquillo, Cristina; Gonzalez, Carmen; Martinez-Castanon, Gabriel; Martinez-Gutierrez, Fidel (2015)

ANTI-BIOFILM AND CYTOTOXICITY ACTIVITY OF IMPREGNATED DRESSINGS WITH SILVER NANOPARTICLES

Mater Sci Eng C Mater Biol Appl 49():604-611
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Infections arising from bacterial adhesion and colonization on chronic wounds are a significant healthcare problem. Silver nanoparticles (AgNPs) impregnated in dressing have attracted a great deal of attention as a potential solution. The goal of the present study was to evaluate the anti-biofilm activities of AgNPs impregnated in commercial dressings against Pseudomonas aeruginosa, bacteria isolated of chronic wounds from a hospital patient. The antimicrobial activity of AgNPs was tested within biofilms generated under slow fluid shear conditions using a standard bioreactor. A 2-log reduction in the number of colony-forming units of P. aeruginosa was recorded in the reactor on exposure to dressing impregnated with 250ppm of AgNPs, diameter 9.3±1.1nm, and also showed compatibility to mammalian cells (human fibroblasts). Our study suggests that the use of dressings with AgNPs may either prevent or reduce microbial growth in the wound environment, and reducing wound bioburden may improve wound-healing outcomes.