Jesús Aguirre Linares

Intereses de Investigación

Our research is directed to understand the signaling roles of reactive oxygen species (ROS), such as H2O2, in cell physiology and differentiation [1-7]. Mitochondria [8] and NADPH oxidase enzymes [9] are the most important sources of ROS in eukaryotic cells and play critical roles in general cell physiology and cell differentiation [1,6,10-12]. A lack of mitochondrial division results in severe growth and developmental defects in the fungus Aspergillus nidulans [11], while imbalances in mitochondrial fusion and fission are related to neurodegenerative diseases, like Alzheimer and Parkinson [15].

We reported that H2O2 induces dramatic changes in mitochondrial morphology [11,17], including mitochondrial constriction, division and outer membrane remodeling, and that constriction and division require mitochondrial depolarization, calcium and the endoplasmic reticulum-mitochondria encounter structure complex [16]. We also identified the dynamin-like protein DnmA and its putative receptor FisA as essential for mitochondrial division and partially required for peroxisome division. The lack of these proteins produces increased mitochondrial ROS levels and notable defects in asexual and sexual differentiation [11].

1. Aguirre, J.; Rios-Momberg, M.; Hewitt, D.; Hansberg, W. Reactive oxygen species and development in microbial eukaryotes. Trends in microbiology 2005, 13, 111-118, doi:10.1016/j.tim.2005.01.007.
2. Cano-Dominguez, N.; Bowman, B.; Peraza-Reyes, L.; Aguirre, J. Neurospora crassa NADPH Oxidase NOX-1 Is Localized in the Vacuolar System and the Plasma Membrane. Frontiers in Microbiology 2019, 10, doi:ARTN 182510.3389/fmicb.2019.01825.
3. Hansberg, W.; Aguirre, J. Hyperoxidant states cause microbial cell differentiation by cell isolation from dioxygen. Journal of theoretical biology 1990, 142, 201-221.
4. Mendoza-Martinez, A.E.; Lara-Rojas, F.; Sanchez, O.; Aguirre, J. NapA Mediates a Redox Regulation of the Antioxidant Response, Carbon Utilization and Development in Aspergillus nidulans. Front Microbiol 2017, 8, 516, doi:10.3389/fmicb.2017.00516.
5. Lara-Rojas, F.; Sanchez, O.; Kawasaki, L.; Aguirre, J. Aspergillus nidulans transcription factor AtfA interacts with the MAPK SakA to regulate general stress responses, development and spore functions. Mol Microbiol 2011, 80, 436-454, doi:10.1111/j.1365-2958.2011.07581.x.
6. Lara-Ortiz, T.; Riveros-Rosas, H.; Aguirre, J. Reactive oxygen species generated by microbial NADPH oxidase NoxA regulate sexual development in Aspergillus nidulans. Mol Microbiol 2003, 50, 1241-1255.
7. Mendoza-Martínez, A.E.; Sánchez, O.; Aguirre, J. The role of peroxiredoxins PrxA and PrxB in the antioxidant response, carbon utilization and development in Aspergillus nidulans. Fungal Biology 2022.
8. Nunnari, J.; Suomalainen, A. Mitochondria: In Sickness and in Health. Cell 2012, 148, 1145-1159, doi:10.1016/j.cell.2012.02.035.
9. Aguirre, J.; Lambeth, J.D. Nox enzymes from fungus to fly to fish and what they tell us about Nox function in mammals. Free Radical Bio Med 2010, 49, 1342-1353, doi:10.1016/j.freeradbiomed.2010.07.027.
10. Cano-Dominguez, N.; Alvarez-Delfin, K.; Hansberg, W.; Aguirre, J. NADPH oxidases NOX-1 and NOX-2 require the regulatory subunit NOR-1 to control cell differentiation and growth in Neurospora crassa. Eukaryot Cell 2008, 7, 1352-1361, doi:10.1128/Ec.00137-08.
11. Garrido-Bazan, V.; Pardo, J.P.; Aguirre, J. DnmA and FisA Mediate Mitochondria and Peroxisome Fission, and Regulate Mitochondrial Function, ROS Production and Development in Aspergillus nidulans. Frontiers in Microbiology 2020, 11, doi:ARTN 837
10.3389/fmicb.2020.00837.
12. Seo, B.J.; Yoon, S.H.; Do, J.T. Mitochondrial Dynamics in Stem Cells and Differentiation. Int J Mol Sci 2018, 19, doi:10.3390/ijms19123893.
13. Salazar-Roa, M.; Malumbres, M. Fueling the Cell Division Cycle. Trends Cell Biol 2017, 27, 69-81, doi:10.1016/j.tcb.2016.08.009.
14. Wakabayashi, J.; Zhang, Z.Y.; Wakabayashi, N.; Tamura, Y.; Fukaya, M.; Kensler, T.W.; Iijima, M.; Sesaki, H. The dynamin-related GTPase Drp1 is required for embryonic and brain development in mice. J Cell Biol 2009, 186, 805-816, doi:10.1083/jcb.200903065.
15. Atkins, K.; Dasgupta, A.; Chen, K.H.; Mewburn, J.; Archer, S.L. The role of Drp1 adaptor proteins MiD49 and MiD51 in mitochondrial fission: implications for human disease. Clin Sci (Lond) 2016, 130, 1861-1874, doi:10.1042/CS20160030.
16. Garrido-Bazan, V.; Aguirre, J. H2O2 Induces Calcium and ERMES Complex-Dependent Mitochondrial Constriction and Division as Well as Mitochondrial Outer Membrane Remodeling in Aspergillus nidulans. J Fungi (Basel) 2022, 8, doi:10.3390/jof8080829.
17. Jaimes-Arroyo, R.; Lara-Rojas, F.; Bayram, O.; Valerius, O.; Braus, G.H.; Aguirre, J. The SrkA Kinase Is Part of the SakA Mitogen-Activated Protein Kinase Interactome and Regulates Stress Responses and Development in Aspergillus nidulans. Eukaryot Cell 2015, 14, 495-510, doi:10.1128/Ec.00277-14.

Trayectoria Profesional

Investigador Titular C

Formación Académica

Doctorado en Ivestigación Biomédica Básica, UNAM, México (1988)

Integrantes del Laboratorio

Alma Olivia Sánchez González

Estudiantes