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Moran, J; Pasantes Morales, H; SanchezOlea, R; PENA, C (1993)

INHIBITION OF VOLUME REGULATION AND EFFLUX OF OSMOREGULATORY AMINO-ACIDS BY BLOCKERS OF CL- TRANSPORT IN CULTURED ASTROCYTES

NEUROSCI LETT 156():141-144
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Regulatory volume decrease (RVD) in astrocytes was inhibited by the Cl- exchanger blockers 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), dipyridamole and niflumic acid but not by the Cl- channel inhibitors diphenylamine-2-carboxylate (DPC) and anthracene-9-carboxylate (9-AC). The volume activated efflux of [H-3]taurine and [H-3]D-aspartate (as marker for glutamate) was similarly affected by these compounds. However, neither RVD nor osmolyte fluxes were significantly reduced by removal of external Cl-, suggesting that an anion exchanger activity is not required for the volume regulatory process. Alternatively, these results suggest that the anion exchanger molecule may function as an unidirectional Cl- channel possibly permeable also to amino acids.