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Hansberg, W; Garay-Arroyo, A; Lledias, F; Covarrubias, AA (2003)

CU, ZN-SUPEROXIDE DISMUTASE OF SACCHAROMYCES CEREVISIAE IS REQUIRED FOR RESISTANCE TO HYPEROSMOSIS

FEBS LETT 539():68-72
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Here we analyzed the role of the antioxidant response in Saccharomyces cerevisiae adaptation to hyperosmotic stress. We show that Cu,Zn-superoxide dismutase (SOD1) plays a fundamental role in this adaptation process since under hyperosmosis SOD1 mutants lead to high protein oxidation levels and show a sensitive phenotype, which is reversed by the addition of N-acetylcysteine to the medium. Pretreatment with MnCl2, a superoxide scavenger, improves the survival of the sod1 strain upon hyperosmosis. Additionally, we show that upon hyperosmotic shock there is a small and transient increase in SOD1 transcript levels, regulated by the protein kinase A-cAMP and SKN7 pathways. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.